Whitley H155 HEPA Hypoxystation – Hypoxic Chamber
The Whitley H155 HEPA Hypoxystation stands out as the largest and most spacious hypoxic chamber among the Whitley Workstation series. With a substantial internal capacity of 1,900 liters, it offers versatile accommodation for a range of equipment, including live cell imaging devices and microscopes. This workstation provides an expansive workspace tailored to meet diverse research requirements.
Description
Hypoxic Chamber
The Whitley H155 HEPA Hypoxic Chamber is a large-capacity controlled environment workstation designed for cell culture, live-cell imaging, incubation, and analysis under precise hypoxic conditions. With a usable internal capacity of 1,900 litres, it provides ample working space for laboratory equipment while maintaining controlled oxygen levels and a clean internal environment.
What is a Hypoxic Chamber?
A hypoxic chamber is a controlled laboratory environment designed to maintain oxygen levels below normal atmospheric conditions. It allows researchers to culture, manipulate, incubate, and analyse cells under controlled oxygen, carbon dioxide, temperature, and humidity conditions without repeatedly exposing samples to ambient air.

Key Features of the Whitley H155 HEPA Hypoxystation
- 1,900-litre internal capacity
- Large working and incubation area
- Integrated HEPA filtration system
- Atmospheric cleanliness exceeding ISO 14644 Class 3 requirements
- Two rapid 16-litre airlocks
- Independent airlock operation
- Approximately 60-second airlock cycle
- Suitable for live-cell imaging equipment and microscopes
- Easy pipetting and cell manipulation inside controlled conditions
- Precise environmental control
Applications of Hypoxic Chamber
The Whitley H155 HEPA Hypoxystation can support applications such as:
- Cell Culture
- Cancer Research
- Neurology Research
- Cardiovascular Research
- Live-Cell Imaging
- Cell Biology
- Tissue Culture
- Hypoxia Research
- Cellular Analysis
Hypoxic Chamber vs Hypoxic Workstation
| Hypoxic Chamber | Hypoxic Workstation |
|---|---|
| Controlled low-oxygen environment | Controlled low-oxygen working environment |
| Used for cell and tissue research | Designed for manipulation, culture and analysis |
| Maintains controlled atmospheric conditions | Allows researchers to work inside the controlled environment |
| May refer broadly to the enclosed system | Often used for integrated laboratory workstations |
The terms hypoxic chamber and hypoxic workstation are often used interchangeably in laboratory research, although a workstation generally emphasizes the ability to perform hands-on cell manipulation within the controlled environment.
Why HEPA filtration matters?
The H155 HEPA Hypoxystation incorporates an internal HEPA filtration system to maintain high levels of atmospheric cleanliness inside the workstation. The system is designed to achieve cleanliness levels exceeding ISO 14644 Class 3 requirements, supporting sensitive cell culture and laboratory applications.
Rapid Airlocks for Controlled Sample Transfer
Two independent 16-litre airlocks allow materials and samples to be transferred into and out of the hypoxic chamber while helping maintain the controlled environment. Each airlock completes its cycle in approximately 60 seconds.
Equipment That Can Be Accommodated
The spacious H155 chamber can accommodate laboratory equipment such as:
- Live-cell imaging systems
- Microscopes
- Culture equipment
- Incubation equipment
- Other compatible laboratory instruments
What is a hypoxic chamber?
A hypoxic chamber is a controlled laboratory environment used to maintain reduced oxygen conditions for cell culture and other research applications.
What is the capacity of the Whitley H155 HEPA Hypoxystation?
The H155 HEPA Hypoxystation has a usable internal capacity of approximately 1,900 litres.
What is a hypoxic chamber used for?
It is used for applications such as cell culture, hypoxia research, live-cell imaging, cancer research, neurology, and cardiovascular studies.
Does the H155 have HEPA filtration?
Yes. The H155 HEPA includes an internal HEPA filtration system designed to provide atmospheric cleanliness exceeding ISO 14644 Class 3 requirements.
How many airlocks does the H155 have?
The workstation has two independent 16-litre airlocks, with each cycle taking approximately 60 seconds.
Can microscopes be used inside the hypoxic chamber?
Yes. The large internal space is designed to accommodate equipment such as live-cell imaging devices and microscopes.
Who supplies the Whitley H155 HEPA Hypoxystation in India?
Lab Technologies India supplies the Whitley H155 HEPA Hypoxystation and provides laboratory equipment solutions for life-science and research applications.
Overview
The generous internal height facilitates easy pipetting and all cell manipulations can be performed without removing them from your required hypoxic conditions. The H155 HEPA is equipped with the Whitley Internal HEPA Filtration System. Levels of atmospheric cleanliness inside the workstation exceed the requirements of ISO 14644 Class 3 and we can provide proof of atmospheric cleanliness.
This workstation provides precise environmental control whilst leaving plenty of room to work, incubate and conduct analysis. Two rapid 16 litre airlocks prevent compromising conditions inside the chamber when you are entering and exiting. The airlocks operate independently and take only 60 seconds to run a cycle. Each airlock can accommodate 10 x 500ml Duran bottles.
If you need to aspirate biological liquid waste from inside the chamber, order with a Vacuum Take-off Port and the Whitley AsPROvac for the perfect package.
- Precise control of oxygen, carbon dioxide and nitrogen – provides flexibility in your research and confidence in your results.
- The Whitley Internal HEPA Filtration System with optional Enhanced Biological Containment.
- Two removable fronts (each fitted with two patented oval ports) facilitate the transfer of large items of equipment for use inside the chamber.
- Innovative, full colour touch screen that is Ethernet-enabled for remote access. Now you can log into your workstation when you are away from the laboratory.
- Fully automated oxygen calibration routine (patent pending) – activated via the touchscreen with no need to touch or remove the sensor – saves time, no risk of user error and ensures the accuracy of your results.
- Other options include double internal power socket, refrigeration, front loading single sample entry system, cable glands and storage trays.
Specifications
| Capacity Litres | 600 |
| Port Airlock Capacity | Accommodates up to 44 x 96 well plates or 84 x T25s |
| Airlock Size (litres) | 12 |
| Porthole System | Manual Ports |
| Gas Supplies | Air and Nitrogen |
| Footswitch | Wireless |
| Auto Sleeve Gassing | Available |
| Internal Mains Sockets | Included |
| Storage Tray | Available |
| Inspection Lamp | Available |
| Data Logging | Available |
| Airlock Cycle Time (seconds) | 60 |
| Extra Cable Glands | Available |
| Extra Cable Glands | Available |
| Microscope | Contact Us |
| Automatic Dehumidifier | Included |
| Removable Front | Included |
| Trolley Available | Included |
| Co2 Monitoring | Included |
| Vacuum Take Off | Available |
| O2 Profiling | Available |
| Temperature Range | 4°C above ambient up to 45°C |
| Touchscreen Control | Yes |
| Remote Access | Included |
| HEPA | As Standard |
| Dimensions (W/D/H mm) | 2875 / 1056 / 1063 |
| Weight (lbs/kgs) | 771 / 350 |
Options
| A00006 | Electronic Gas Leak Detector |
| A01554 | Air Regulator |
| A01747 | Two Stage CO2 Regulator |
| A01748 | Nitrogen Regulator |
| A02800 | Combined IOQ Documentation |
| A02945 | Cable Gland |
| A06102 | Vacuum Take-off Port |
| A06107 | Gas Sample Port |
| A06108 | Data Logging with Oxygen Profiling |
| A06109 | Data Logging |
| A06114 | Internal Storage Tray |
| A06116 | Automatic Sleeve Gassing System |
| A06128 | Large Cable Gland |
| A06135 | Multiple Cable Port |
| A07200 | Data Logging |
| A07208 | Front Loading 9cm Letterbox |
| A07211 | Whitley Workstation Spotlight LH |
| A07218 | Whitley Enhanced Biological Containment System |
| A07219 | Whitley Workstation Spotlight RH |
| A07951 | AsPROvac and Vacuum Take-off Port Option |
| A08102 | 45° Free Standing Double Internal Power Socket |
| A08167 | Multiple Cable Port (Extra Large) |
| A08502 | Gas Control Alarm and Email Alert Option – Hypoxic |
| A08757 | 135 Refrigeration Unit 230V |
| A08974 | 440 x 400mm Platform Assembly |
| A08975 | 520 x 520mm Platform Assembly |
| A08982 | 135/155 Turntable Accessory |
| A09372 | 135/155 Pipette Hanger |
| A09600 | 135 Heat Removal System 230V |
| L00040 | Temperature Mapping |
| L00050 | Single Point Temperature Calibration |

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